FlowFuel Trifecta Testing™
Stop Guessing.
Start Knowing What Your Body Actually Needs.
Three precision functional wellness tests. One comprehensive protocol. Which supplements are worth your money you — not a formula built for everyone.
In short FlowFuel.Pro offers three at-home wellness assessments: metabolomics from a urine sample, GI from a stool sample and genomic from a cheek swab. Each is read with a nutritionist and turned into a written plan. One assessment is $495 and FlowFuel Trifecta Testing is $2,500. Not available in New York State or U.S. territories.
How testing works
From kit to written plan in five steps. 49 seconds.
The Problem
Most People Supplement by Guessing. Their Bodies Pay the Price.
You train, eat clean, track everything. But the afternoon energy crash still hits. Sleep still doesn't restore. Focus slips. You add another supplement. Nothing changes.
The issue isn't effort. It's data. Without knowing what's actually happening inside your metabolism, gut, and cells, you're optimizing blindfolded — burning money on supplements your body may not even be able to use.
Energy That Won't Stick
Krebs cycle inefficiencies and mitochondrial bottlenecks don't show on standard labs — but they drain you daily. Your metabolomics report helps show where the breakdown may be.
Brain Fog & Mood Drift
Serotonin, dopamine, and GABA are measurable. Your neurotransmitter metabolites tell us where the imbalance is and which nutrients may support better balance.†
Gut Issues That Keep Returning
Bloating, reactivity, inconsistent digestion — these signal microbial imbalance that your Trifecta GI Assessment maps down to the DNA level.†
Supplements That Never Work for You
If your MTHFR or detox SNPs carry variants, standard supplements may not suit you. Your Genomic test helps show which forms may suit your body.
The FlowFuel Difference
Standard Wellness vs. Biomic Optimization
Standard Wellness
- Generic "Reference Range" bloodwork
- One-size-fits-all supplement formulas
- Guessing based on symptoms alone
- No genetic or metabolic context
- Short-term "fixes" that don't stick
FlowFuel Biomic Optimization
- Deep-dive Metabolomic & Genomic data
- N-of-1 protocol built for your unique DNA
- Data-driven decisions, zero guessing
- A whole-system view of your results
- A plan you can keep following
Three tests. One plan.
What each test looks at and how they are read together. 40 seconds.
Three Tests. One Complete Picture of Your Biology.
FlowFuel Trifecta Testing is FlowFuel.Pro's core functional wellness assessment — three complementary tests that map your metabolism, your gut microbiome, and your genetic blueprint simultaneously.
Where most wellness programs give general recommendations, Trifecta Testing tells us precisely which targeted FlowFuel supplements your unique biochemistry is most likely to respond to — and which you simply don't need.
Every result is reviewed by our clinical nutritionist and the doctors on our board of advisers. You receive a full personalized protocol — not a raw report. Reviewed and personally signed off by Jamie Mentzer, founder of FlowFuel.Pro and BioFlow.Pro.
Your Three-Test Protocol
Trifecta Metabolomics Assessment™
Urine · Maps energy, neurotransmitters, detox, B-vitamin status, and microbial metabolites across 60+ biomarkers.
Trifecta GI Assessment™
Stool · DNA/PCR analysis of your full gut microbiome — bacteria, fungi and digestive function markers.
Trifecta Genomic Assessment™
Cheek swab · One-time genetic blueprint across 8 key health domains including methylation, detox, and cognitive SNPs.
The Three Tests
What Each Test Reveals — and Why It Matters
Trifecta Metabolomics Assessment™
- →Energy Metabolism — Krebs cycle, glycolysis, fatty acid oxidation, ketone status
- →B-Vitamin Status — B1, B2, B3, B5, B6, B12, folate, and biotin functional markers
- →CNS Neurotransmitters & Cortisol — serotonin turnover, dopamine, GABA, free cortisol†
- →Toxic Impact Markers — Phase I & II liver detox load, oxidative stress, mold exposure signals
- →Gut Microbial Metabolites — bacterial and fungal population signatures via urine organic acids
Trifecta GI Assessment™
- →Beneficial bacteria populations — Lactobacillus, Bifidobacterium, Akkermansia and more
- →Opportunistic and pathogenic bacteria — quantified by DNA, not culture estimates
- →Fungal presence — including Candida species and other fungi
- →Digestive function — enzyme activity and nutrient absorption markers
- →Gut-wall integrity signals — inflammatory and immune response markers
- →Parasites and protozoa — quantified by DNA
Trifecta Genomic Assessment™
- →Methylation — MTHFR and related SNPs affecting B-vitamin use and detoxification
- →Detoxification — CYP450 variants and Phase I/II liver detox capacity
- →Cognitive Health — genetic markers affecting focus, memory, neuroinflammation risk
- →Inflammatory Status — variants modulating your inflammatory response profile
- →Endocrine Health — hormone metabolism and receptor sensitivity genetics
- →Gastrointestinal Health — genetic factors in gut function and microbiome resilience
- →Structural Health — collagen synthesis and mineral absorption genetics
- →Aging & Cellular Repair — oxidative stress and longevity-related genetic factors
From Order to Protocol in 4 Steps
Order Your Test
Choose your test or bundle. Your kit ships with full instructions and prepaid return packaging.
Collect at Home
Simple at-home collection — urine, stool, or cheek swab depending on the test. Takes minutes.
Lab & Clinical Review
Accredited lab analyzes your sample. Our clinical nutritionist and board of advisers review alongside your full health history.
Receive Your Protocol
Jamie personally reviews and signs off. You receive a precise, targeted FlowFuel supplement protocol built specifically for your results.†
Clinical Standards
Every Protocol Reviewed Before It Reaches You
Trifecta Testing is not a self-service report. Every result goes through a multi-layer clinical review before your protocol is delivered — because a supplement plan is only as valuable as the expertise behind it.
Testing is conducted by CLIA-certified, accredited laboratories. Results are interpreted through a systems-biology approach — connecting pathway patterns, not just flagging isolated out-of-range values.
CLIA-Certified Laboratory Analysis
All tests processed by accredited, CLIA-certified labs. Research-grade analysis — not reference-range-only interpretation.
Doctors on Our Board of Advisers
Licensed physicians on the FlowFuel.Pro board of advisers review every protocol for clinical accuracy and safety before finalization.
Clinical Nutritionist Integration
Your prior labs, bloodwork, and health history are reviewed by our certified clinical nutritionist — your protocol fits your full picture, not just today's test.
Personal Review by Jamie Mentzer
Founder of FlowFuel.Pro and BioFlow.Pro. Jamie personally reviews and signs off every Trifecta Testing protocol before delivery.
Not Sure Where to Start?
Do You Actually Need Coaching?
Take this 60-second assessment to find out if you should just buy the tests, or if you need the integration support of our 3-month coaching curriculum.
The True Cost of Guessing
Interactive ROI Calculator
Many people spend more than they realize on trial and error. Use this calculator to estimate what you have spent on supplements and therapies chosen without data.
Acupuncture, IV drips, random wellness gadgets.
Total Spent Guessing
$15,000
YOUR ESTIMATE
$9,000
Cost of FlowFuel Trifecta Testing
$2,500
DIFFERENCE
$6,500
By stopping the guesswork and getting real data.
Illustration only, based on the numbers you enter. Your costs will differ.
Pricing & Programs
Start With What Your Body Actually Needs
Most people who do one test wish they had done all three. The protocols compound — your metabolism, gut, and genome tell a connected story.
Complete Three-Test Protocol
FlowFuel Trifecta Testing
All 3 lab assessments, one protocol and 3 months of coaching. Three months of dedicated coaching — one session per week with your assigned coach, one session per month directly with Jamie. This is where most people start and where results begin compounding fast.
Testing: $2,500 vs $2,985 for three Single Test + 3-Month Coaching packages
Get the FlowFuel Trifecta Testing — $2,500Your biology shifts as your protocol takes effect. For the year after your initial test, retests of your Metabolomics, GI, and Hormone assessments are $300 each — so you can dial in and update your supplementation during transformation. (Your Genomic DNA blueprint never changes, so it never needs retesting.)
Subscribe & Save: Enroll in a retest subscription and buy retest packs for $250 each — the lowest retest price available.
Everything included:
- Trifecta Metabolomics, GI and Genomic Assessments™
- One unified protocol across all three results
- Clinical nutritionist review of your full health history
- Jamie's protocol review and sign-off
- In-person results meeting with Jamie (Laguna Beach)
- 3 months of coaching: 1 session a week with your coach
- 1 session a month with Jamie
- FlowFriends community
Add testing to your Flow plan
Training with BioFlow.Pro? Add FlowFuel Trifecta Testing to any Flow level in the same checkout. Flow Deep plus testing is Flow + Fuel ($7,500).
Add testing to my Flow planSingle Test with Coaching
Single Test + 3-Month Coaching
Start with the test most relevant to your biggest concern — with the full clinical review layer, an in-person kickoff with Jamie, and 3 months of dedicated coaching support to implement your protocol.
Note: without all three tests, your protocol will address one dimension. Most clients add remaining tests within 90 days once they experience the results.
Choose: Metabolomics • GI Assessment • or Genomic
Everything included:
- Your chosen Trifecta test + lab analysis
- Personalized report and protocol
- Clinical nutritionist review
- Jamie's personal protocol review
- In-person kickoff with Jamie (Laguna Beach)
- 3 months of coaching: 1 session a week with your coach
- 1 session a month with Jamie
- FlowFriends community
Build Your Own
Custom Bundle Calculator
Select the tests and add-ons you need. Note: FlowFuel Trifecta Testing (3 tests + coaching) automatically applies a bundle discount.
1. Select Tests ($495 each)
2. Add Coaching? (+$500 per test)
3. Hormone Add-on (+$500)
You never pay twice
You never pay twice. Move up within 60 days of your last purchase and everything you've paid counts toward the higher level.
The 3-Month Coaching Curriculum
A structured, data-driven approach to integrating your lab results and locking in sustainable habits.
Month 1: Discovery & Integration
- Deep dive into lab results and health history
- Customized supplement and nutrition protocol delivery
- Establishing baseline metrics and daily non-negotiables
Month 2: Adaptation & Optimization
- Bi-weekly check-ins to monitor protocol adherence
- Troubleshooting roadblocks and adjusting dosages
- Biofeedback tracking and lifestyle integration
Month 3: Mastery & Continuity
- Evaluating subjective and objective shifts in performance
- Preparing for re-testing and tracking progression
- Transitioning to sustainable, long-term maintenance
Flow VIP
The top of the Flow Ladder: everything in Flow + Fuel, with Jamie. By application.
Flow + Fuel ($7,500) and a Jamie Kickoff ($1,000) cost $8,500 on their own. Flow VIP Elite adds 12 months of weekly 1:1 with Jamie, quarterly retests and priority support for $1,500 more in your first year.
Flow VIP Elite
Weekly 1:1 with Jamie
- ✓Everything in Flow + Fuel ($7,500 on its own)
- ✓Jamie Kickoff included
- ✓Weekly 1:1 sessions with Jamie for 12 months
- ✓Quarterly retests
- ✓Priority support
Flow VIP Concierge
Direct priority access to Jamie
- ✓Everything in Flow VIP Elite
- ✓Customized Nutrition Formula ($5,000 on its own)
- ✓$2,500 supplement credit
- ✓VIP event access
Flow VIP In-Home
On-demand concierge
- ✓Everything in Flow VIP Concierge
- ✓$10,000 supplement credit
- ✓In-home setup and assessment
- ✓Sourced and vetted local trainers
- ✓Coordinated Flow Team and DNA Concierge
Want time with Jamie without VIP?
Jamie Kickoff · $1,000 · Add to any Flow level
- ✓Deep-dive consultation with Jamie
- ✓Two follow-up integration sessions
- ✓Personalized action plan
- ✓Email support
Request More Information
Get the complete breakdown of our testing protocols.
Order FlowFuel Trifecta Testing
All 3 lab assessments, one protocol and 3 months of coaching ($2,500)
Referred by a doctor? Enter your partner code at checkout for special pricing.
Frequently Asked Questions
† These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease. All laboratory testing is ordered and authorized through an independent licensed physician network, as required by law. Your order is reviewed by a licensed provider before your kit ships.
Trifecta Testing™ assessments are currently not available to residents of New York State or U.S. territories due to regulations governing direct-access laboratory testing. Some content and communications are prepared with AI assistance and reviewed by our clinical team prior to publication. Individual results will vary.